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Folate and the Methylation Cycle

5-MTHF donates a methyl group to homocysteine to make methionine, fueling SAM for methylation.

A free, animated folate and the methylation cycle you can read here or embed on any website, from Scrollchart.

Folate and the Methylation Cycle

Folate and the Methylation Cycle5-MTHF donates a methyl group to homocysteine via B12-dependent methionine synthase, fueling SAMFolate cycleMethionine cycleFolateB9 intakeDHFdihydrofolateTHFtetrahydrofolate5,10-methylene-THFserine donates CH25-MTHFvia MTHFRMTHFRMethioninemethyl donor poolSAMS-adenosylmethionineSAHS-adenosylhomocysteineHomocysteineHcyMethionine Synthasecofactor: B12 (methyl-Cbl)B6: transsulfurationHcy to cystathionine to cysteine to GSHSAM donates CH3DNA, neurotransmitters,phospholipids, creatineB9, B12, and B6 cofactors gate the cycle: deficiency raises homocysteine and depletes SAM-driven methylation

Folate to DHF to THF to 5,10-methylene-THF to 5-MTHF, which methylates homocysteine to methionine, then SAM, SAH, homocysteine, restart. Diagram animates the cycle with B12 and B6 inputs.

Good for

  • One-carbon metabolism explainers
  • MTHFR and methylation supplement reviews
  • Homocysteine and cardiovascular risk articles

Source & accuracy

This folate and the methylation cycle is an editorial illustration built to represent the concept accurately. Where it shows figures, they are typical or representative values chosen to make the relationship clear, not a single underlying dataset. The diagram and its explainer are reviewed and maintained centrally, and updated over time as understanding improves.

How 5-MTHF feeds the methylation cycle

Folate (vitamin B9) enters the methylation cycle as 5-methyltetrahydrofolate (5-MTHF), its active circulating form. 5-MTHF donates its methyl group to homocysteine in a reaction that also requires vitamin B12, regenerating methionine. Methionine is then activated to S-adenosylmethionine (SAM), the body's universal methyl donor for DNA, RNA, proteins, neurotransmitters, and phospholipids.

The diagram traces this handoff: folate supplies the one-carbon unit, B12 carries it onto homocysteine, and the cycle keeps SAM topped up. Folate and B12 are interdependent here, which is why a deficiency in one can mask or mimic the other.

Why homocysteine is the readout

When folate (or B12) is insufficient, homocysteine cannot be efficiently recycled to methionine and tends to accumulate, which is why blood homocysteine is used as a functional marker of this cycle. Elevated homocysteine is associated with cardiovascular and other risks, though lowering it with supplements has not consistently translated into better clinical outcomes in trials.

Adequate folate around conception is well established for reducing neural tube defects. This is general educational information, not medical advice; folate status, pregnancy planning, and homocysteine should be managed with a qualified professional.

Embed this diagram

Add this animated folate and the methylation cycle to your own site. Copy one line of HTML, or use the embed builder for theme and sizing options.

Reference

What this is
A free, embeddable, animated folate and the methylation cycle for any website.
Who uses it
Supplement reviewers, Health blogs, Nutrition blogs, Biohacker blogs.
How to embed
Copy one line of HTML. No signup. No watermark. Works in WordPress, Webflow, Ghost, Substack, plain HTML.
File size
iframe embed, ~80 KB gzipped (loads on demand, does not block your page paint).
License
Free forever. Editorial explainer text included; updated centrally over time.

Embed format options

Copy the universal HTML snippet, the WordPress shortcode, or an iframe fallback - see the WordPress plugin page for details. Any format keeps the same Core Web Vitals profile and the same explainer text.

Embed snippet
<div data-scrollchart="folate-methylation" data-scrollchart-v="1"></div>
<script src="https://scrollchart.com/embed.js" async></script>

Frequently asked questions

Where can I get a free animated "Folate and the Methylation Cycle" for my website?
Scrollchart provides "Folate and the Methylation Cycle" as a free, embeddable animated diagram you can add to any website with one line of HTML. No signup is required and there is no watermark. The diagram and its explainer text are served from scrollchart.com, so the embed stays current without any maintenance on your end.
How do I embed a folate and the methylation cycle diagram in a health or wellness blog?
Copy the HTML snippet from the Scrollchart diagram page and paste it into any post or page in your CMS. It works in WordPress, Webflow, Ghost, Substack, and plain HTML without any plugin or account. The diagram renders as animated SVG directly in your page, so search engines can index the accompanying explainer text.